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Registros recuperados : 617 | |
4. | | CASTRO, I. M. de; BASTOS, L. H. P.; ANJOS, M. R. dos; OLIVEIRA, A. C. DE; COSTA, D. M. S. F. DA; CARDOSO, M. H. W. M.; CARVALHO, L. M. J. DE. Multi-residue method of pesticides by UPLC-MS/MS in bivalve mollusks samples as a tool for food quality and safety. Food Science and Technology, v. 43, e116722, 2023. Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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6. | | PEREIRA, M. A. V.; ANDRADE, C. de L. T. de; SARAIVA JÚNIOR, L. A.; SILVA, A. P. F. da; SILVA, W. A. S. da; OLIVEIRA, A. C. de; GUIMARAES, P. E. de O.; TRINDADE, R. dos S. Produtividade de grãos de milho para diferentes níveis de estresse hídrico. In: SEMINÁRIO NACIONAL DE MILHO SAFRINHA, 17., 2023, Campo Grande, MS. Preservar e produzir: anais. Maracaju: Fundação MS, 2023. p. 125-126. Biblioteca(s): Embrapa Milho e Sorgo. |
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8. | | GUIMARÃES, C. T.; MAGALHAES, J. V. de; ANDRADE, C. de L. T. de; MENEZES, C. B. de; GUIMARAES, L. J. M.; MAGALHAES, P. C.; OLIVEIRA, A. C. de; MARTINS, M. Tolerância ao alumínio conferida por genes MATE garante estabilidade de produção em híbridos de sorgo e de milho sob deficiência hídrica. Sete Lagoas: Embrapa Milho e Sorgo, 2023. 11 p. (Embrapa Milho e Sorgo. Circular Técnica, 286). Biblioteca(s): Embrapa Milho e Sorgo. |
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9. | | OLIVEIRA, V. F. de; VENSKE, E.; STAFEN, C. F.; PANIZ, F. P.; PEDRON, T.; PEREIRA, R. M.; MAGALHAES JUNIOR, A. M. de; MAIA, L. C. da; OLIVEIRA, A. C. de; BATISTA, B. L.; PEGORARO, C. Genome-wide association of iron content in rice grains grown in Southern Brazil. Pesquisa Agropecuária Brasileira, Brasília, v. 58, e03203, 2023. Título em português: Associação genômica ampla para teor de ferro em grãos de arroz na região Sul do Brasil. Biblioteca(s): Embrapa Clima Temperado; Embrapa Unidades Centrais. |
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10. | | SANTOS, F. C. dos; REIS, D. P. dos; GOMES, E. A.; LADEIRA, D. de A.; OLIVEIRA, A. C. de; MELO, I. G.; SOUZA, F. F. de; MATTOS, B. B.; CAMPOS, C. N.; OLIVEIRA-PAIVA, C. A. Influence of phosphorus-solubilizing microorganisms and phosphate amendments on pearl millet growth and nutrient use efficiency in different soils types. African Journal of Microbiology Research, v. 16, n. 3, p. 95-103, 2022. Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Solos. |
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11. | | MOURA, S. M. de; FREITAS, E. O.; RIBEIRO, T. P.; PAES-DE-MELO, B.; ARRAES, F. B. M.; MACEDO, L. L. P. de; PAIXÃO, J. F. R.; LOURENCO, I. T.; ARTICO, S.; VALENÇA, D. da C.; SILVA, M. C. M. da; OLIVEIRA, A. C. de; MARCIO ALVES-FERREIRA, M.; SA, M. F. G. de. Discovery and functional characterization of novel cotton promoters with potential application to pest control. Plant Cell Reports, v. 41, p. 1589-1601, 2022. Na publicação: Leonardo Lima Pepino Macedo; Isabela T. Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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12. | | SILVEIRA, T.; FREITAS, K. E. J. de; SANTOS, R. S. dos; OLIVEIRA, A. C. de; BARBIERI, R. L. Variation in palm tree plastidial simple sequence repeats, characterization, and potential use. Crop Breeding and Applied Biotechnology, v. 22, n. 4, e41812241, 2022. Biblioteca(s): Embrapa Clima Temperado. |
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13. | | SIMÃO, E. de P.; RESENDE, A. V. de; GIEHL, J.; GALVÃO, J. C. C.; BORGHI, E.; OLIVEIRA, A. C. de; GONTIJO NETO, M. M. Agronomic responses to the intensification of grain production systems in dryland farming of central Minas Gerais State, Brazil. Brazilian Journal of Agriculture, v. 96, n. 1, p. 277-293, 2021. Biblioteca(s): Embrapa Milho e Sorgo. |
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14. | | MAZZEI, R. R. F.; FREIRE, E.; SERRA, E. G.; MACEDO, J. R. de; OLIVEIRA, A. C. de; BASTOS, L. H. P.; CARDOSO, M. H. W. M. Estudo comparativo das concentrações de agrotóxicos no solo provenientes dos métodos de plantio do tomate convencional, orgânico e sustentável. Brazilian Journal of Development, v. 7, n. 3, p. 22981-23000, mar. 2021. Biblioteca(s): Embrapa Solos. |
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16. | | OLIVEIRA, V. F. de; BUSANELLO, C.; VIANA, V. E.; STAFEN, C. F.; PEDROLO, A. M.; PANIZ, F. P.; PEDRON, T.; PEREIRA, R. M.; ROSA, S. A.; MAGALHAES JUNIOR, A. M. de; OLIVEIRA, A. C. de; BATISTA, B. L.; PEGORARO, C. Assessing mineral and toxic elements content in rice grains grown in southern Brazil. Journal of Food Composition and Analysis, v. 100, 103914, July 2021. Biblioteca(s): Embrapa Clima Temperado. |
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17. | | MAZZEI, J. R. F.; FREIRE, E.; SERRA, E. G.; MACEDO, J. R. de; OLIVEIRA, A. C. de; BASTOS, L. H. P.; CARDOSO, M. H. W. M. Multiresidue method for analysis of 240 pesticides in tomato planting soils by ultra performance liquid chromatography coupled to mass spectrometry. In: SOUTH FLORIDA CONGRESS OF DEVELOPMENT, 1., 2021. Proceedings [...]. Deerfield Beach: South Florida Publishing, 2021. Evento on-line. Biblioteca(s): Embrapa Solos. |
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18. | | ARAUJO, T. T. S.; COSTA, J. M. A. R. da; RIBEIRO, F. DE O. S.; OLIVEIRA, A. C. DE J.; DIAS, J. DO N.; ARAUJO, A. R. DE; BARROS, A. B.; BRITO, M. DA P.; OLIVEIRA, T. M. DE; ALMEIDA, M. P. D.; CASTRO, K. N. de C.; FOGACA, F. H. dos S.; SILVA, D. A. da; SOUZA, B. W. S. DE. Nanoemulsion of cashew gum and clove essential oil (Ocimum gratissimum Linn) potentiating antioxidant and antimicrobial activity. International Journal of Biological Macromolecules, v. 139, part A, p. 100-108, 2021. Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Tabuleiros Costeiros. |
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19. | | MAZZEI, J. R. F.; FREIRE, E.; SERRA, E. G.; MACEDO, J. R. de; OLIVEIRA, A. C. de; BASTOS, L. H. P. Método multirresíduos para análise de 240 agrotóxicos em solos do plantio de tomate por cromatografia líquida de ultra desempenho acoplada à espectrometria de massa. Revista Científica Multidisciplinar Núcleo do Conhecimento, ano 6, v. 8, n. 1, p. 34-67, jan. 2021. Biblioteca(s): Embrapa Solos. |
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20. | | MAZZEI, J. R. F.; FREIRE, E.; SERRA, E. G.; MACEDO, J. R. de; OLIVEIRA, A. C. de; BASTOS, L. H. P.; CARDOSO, M. H. W. M. Pesquisa de campo: uma análise comparativa entre os métodos de plantio convencional, orgânico e sustentável da produção de tomates. Revista Científica Multidisciplinar Núcleo do Conhecimento, ano 6, v. 5, n. 2, 5, p. 125-146, fev. 2021. Biblioteca(s): Embrapa Solos. |
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Registros recuperados : 617 | |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
19/12/2017 |
Data da última atualização: |
21/05/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. |
Afiliação: |
Adriana Novaes dos Reis, UFPA; Moysés dos Santos Miranda, UFPA; Lílian Kátia Ximenes Silva, UNAMA; Aluizio Otavio Almeida da Silva, UFPA; José Silva de Sousa, UFPA; Eziquiel de Morais, IFPA; ALEXANDRE ROSSETTO GARCIA, CPPSE; Sheyla Farhayldes Souza Domingues, UFPA; Joyce Kelly do Rosário da Silva, UFPA; Haroldo Francisco Lobato Ribeiro, UFRA. |
Título: |
Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017. |
DOI: |
10.5433/1679-0359.2017v38n6p3613 |
Idioma: |
Inglês |
Conteúdo: |
For artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. MenosFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evalu... Mostrar Tudo |
Palavras-Chave: |
Powdered Coconut Water. |
Thesaurus NAL: |
cryopreservation; Lippia. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169304/1/REIS-et-al-2016-Comparative-Evaluation-Between-TES-TRIS-and-ACP-112.pdf
|
Marc: |
LEADER 03425naa a2200277 a 4500 001 2082949 005 2019-05-21 008 2017 bl uuuu u00u1 u #d 024 7 $a10.5433/1679-0359.2017v38n6p3613$2DOI 100 1 $aREIS, A. N. dos 245 $aComparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm.$h[electronic resource] 260 $c2017 520 $aFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. 650 $acryopreservation 650 $aLippia 653 $aPowdered Coconut Water 700 1 $aMIRANDA, M. dos S. 700 1 $aSILVA, L. K. X. 700 1 $aSILVA, A. O. A. da 700 1 $aSOUSA, J. S. de 700 1 $aMORAIS, E. de 700 1 $aGARCIA, A. R. 700 1 $aDOMINGUES, S. F. S. 700 1 $aSILVA, J. K. do R. da 700 1 $aRIBEIRO, H. F. L. 773 $tSemina: Ciências Agrárias, Londrina$gv. 38, n. 6, p. 3613-3628, 2017.
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